Levels of HLA-Class I, -A, -B antigens, β₂μ, ICAM-1 and LFA-3 do not correlate with lysis of melanoma cells induced by autologous and allogeneic NK and LAK cells.

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• 作者：Fonsatti ; Ester ; Coral ; Sandra ; Cattarossi ; Ilaria ; Cattelan ; Alessandro ; Gasparollo ; Aldo ; et. al.
• 刊名：Human Immunology
• 出版年：6 -,
• 出版时间：April, 15 1996 -,
• 年：0
• 卷：47
• 期：1-2
• 页码：72
• 全文大小：50.5 K

文摘

Selected, long term cultured, melanoma cell lines are lysed by NK cells while the majority is efficiently lysed by LAK cells. NK and LAK cell-mediated lysis is not HLA-restricted; nevertheless, an inverse correlation between lysis of individual melanoma cell lines by NK and LAK cells and HLA class I antigens expression has been reported. However, the relationship between quantitative expression of the different genes encoded by the HLA class I region and lysis of melanoma cells by autologous and/or allogeneic NK and LAK cells has not been investigated. Using a 4h⁵¹Cr release assay, we analyzed the lysis of ten primary cultures of melanoma cells (analyzed between in vitro passages 5 to 10) by autologous and allogeneic NK and LAK cells. E/T ratios of 100:1 and 20:1 were used for NK and LAK cells and all effector NK cells used efficiently (>30 % ) lysed the NK-sensitive melanoma cell line F0-1 used as control. Values of⁵¹ Cr release were then correlated with cell surface levels of HLA-class I, -A, -B antigens, β₂μ and cell adhesion molecules (CAM) ICAM-1 and LFA-3 analyzed by flow cytometry and quantitated by QIFIKIT. Two of the melanoma cells investigated had a complete loss of HLA class I antigens expression but were positive for ICAM-1 and LFA-3. The remaining cell lines showed an heterogeneous expression of investigated antigens. Mean values of quantitative expression were: 51700±32100 (HLA class I), 73400±45700 (HLA-A) and 14600±12900 (HLA-B) 72100±41300 (β₂μ), 45300±31100 (ICAM-1) and 36500±43400 (LFA-3). The mean values of ⁵¹Cr release were 4.4±3.5 % (range 0-10 % ) and 5.7±5 % (range 0-16 % ) with autologous and allogeneic NK cells and were 59.6±15.2 % (range 40-78 % ) and 46.4±19.6 % (range 22-76 % ) with autologous and allogeneic LAK cells. Simple linear regression analysis demonstrated no statistically significant correlation between the extent of lysis of melanoma cells and their level of expression of either of the gene products of the HLA class I region, neither by autologous nor by allogeneic NK and LAK cells. Similar results were obtained after more than 30 in vitro passages of melanoma cells investigated in this study. Our results strongly suggest that the sensitivity of melanoma cells to NK and LAK cell-mediated lysis does not depend on the quantitative expression of HLA class I antigens or ICAM-1 or LFA-3 or on the length of in vitro culture. Additional cell membrane molecules, acting individually or in possible association with HLA class I antigens or CAM, may be relevant for melanoma cell lysis by non HLA-restricted cytotoxic cells.