Towards next-generation sequencing analytics for foodborne RNA viruses: Examining the effect of RNA input quantity and viral RNA purity
详细信息 查看全文
- 作者：Zhihui Yang ; Susan R. Leonard ; Mark K. Mammel ; Christopher A. Elkins ; Michael Kulka ; michael.kulka@fda.hhs.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：Detection ; Foodborne viruses ; RNA isolation ; Next generation sequences
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：236
- 期：Complete
- 页码：221-230
- 全文大小：1334 K
文摘
- •
Next generation sequencing successfully applied to low copy number viral RNA.
- •
Viral RNA quantity and purity impact sequencing read depth and coverage.
- •
Confirmed single nucleotide changes identified over a range of viral RNA quantity.