Optimization of spectral light quality for growth and product formation in different microalgae using a continuous photobioreactor

详细信息    查看全文

• 作者：Sascha Baer ; ¹ ; ^{sascha.baer@fau.de" class="auth_mail" title="E-mail the corresponding author} ; Martin Heining¹ ; Philipp Schwerna¹ ; Rainer Buchholz ; Holger Hü ; bner
• 关键词：APC ; allophycocyanin ; CDW ; cell dry weight ; LEDs ; light-emitting diodes ; LHCI ; light-harvesting complex I ; ODλ ; optical density at a certain wavelength λ ; PC ; phycocyanin ; PE ; phycoerythrin ; PFD ; photon flux density ; PSI/II ; photosystem I ; II respectively ; PSM ; photobioreactor screening module ; RGB-LED ; red green blue-light-emitting diodes
• 刊名：Algal Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：14
• 期：Complete
• 页码：109-115
• 全文大小：1122 K

文摘

Many parameters for microalgae cultivation are not fully optimized so far. Even though light is one important process parameter, little research is done regarding its spectral composition. In contrast to other published data, mostly with monochromatic or dichromatic illumination, the objective of this study was to investigate the influence of a broad range of different light qualities to the productivity of cell dry weight as well as the productivity of their phycobiliproteins phycoerythrin or phycocyanin, by keeping the overall light intensity constant. Instead of cultivating in batch mode, cultivations were done in continuous mode in closed, thermal sterilizable photobioreactors. A total of 37 light quality conditions are shown in this work. In case of Chlamydomonas reinhardtii a composition of α_red : α_green : α_blue of 80:10:10 resulted in the highest biomass productivity, with a value of 252 mg·L^− 1·d^− 1. A maximum biomass productivity is achieved for Galdieria sulphuraria at pure red light (304 mg·L^− 1·d^− 1), whereas the highest phycocyanin productivity is reached at a ratio of α_red : α_green : α_blue of 60:0:40 (4.99 mg·L^− 1·d^− 1). The highest biomass and phycoerythrin productivity was achieved for Porphyridium purpureum at α_red : α_green : α_blue of 40:40:20, with values of 312 mg·L^− 1·d^− 1 and 16.93 mg·L^− 1·d^− 1, respectively. The collected data show clearly the significance to investigate the right mixture of light for every microalgal strain and the desired product of interest individually.