Combination of MIDGE-Th1 DNA vaccines with the cationic lipid SAINT-18: Studies on formulation, biodistribution and vector clearance
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- 作者：Anne Endmanna ; endmann@mologen.com" class="auth_mail ; Detlef Oswalda ; Oliver Riedea ; Eduard G. Talmanb ; Roelien E. Vosb ; Matthias Schroffa ; Christiane Kleussa ; Marcel H.J. Ruitersb ; c ; Christiane Juhlsa
- 关键词：MIDGE-Th1 ; DNA vaccine ; Biodistribution ; SAINT-18 ; Transfection reagent ; Cationic lipid
- 刊名：Vaccine
- 出版年：5 June 2014
- 年：2014
- 卷：32
- 期：27
- 页码：3460-3467
- 全文大小：873 K
文摘
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MIDGE-Th1/SAINT-18 lipoplexes were characterized in vitro and in vivo.
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Transfection efficiency of lipoplexes increased with SAINT-18 amount in vitro.
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Lipoplexes containing lower amount of SAINT-18 were most immunogenic in mice.
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Formulation with SAINT-18 resulted in better DNA uptake into draining lymph nodes.
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MIDGE-Th1/SAINT-18 lipoplexes were well tolerated in mice and rats.