Improved detection of cutaneous human papillomavirus DNA by single tube nested ‘hanging droplet’ PCR

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• 作者：Forslund ; Ola ; Ly ; Hoang ; Higgins ; Geoff
• 关键词：HPV ; Skin tumour ; Nested PCR
• 刊名：Journal of Virological Methods
• 出版年：2003
• 出版时间：June 30, 2003
• 年：2003
• 卷：110
• 期：2
• 页码：129-136
• 全文大小：395 K

文摘

A single tube nested ‘hanging droplet’ PCR was developed for detection of cutaneous human papillomavirus (HPV) DNA of the phylogenetic group B1. The nested PCR was compared with a single round PCR method by testing 56 fresh biopsies from Australian skin tumour patients. HPV DNA was detected in 64 % (36/56) of the biopsies by nested PCR and in 30 % (17/56) by single round PCR (P<0.001). HPV DNA was more often detected by nested PCR than by single round PCR in basal cell carcinoma [62 % (16/26) vs. 19 % ; (5/26); P