Differences in transcriptional activity of cutaneous human papillomaviruses

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• 作者：Nataš ; a Vasiljević ; Lone Nielsen ; Geoff Doherty ; Joakim Dillner ; Ola Forslund ; Bodil Norrild
• 关键词：Cutaneous HPV ; URR ; Promoter ; UV-B ; Differentiation
• 刊名：Virus Research
• 出版年：2008
• 出版时间：November 2008
• 年：2008
• 卷：137
• 期：2
• 页码：213-219
• 全文大小：806 K

文摘

The interaction between UV-B irradiation and cutaneous human papillomaviruses (HPV) has been suggested to be of relevance for the development of non-melanoma skin cancers. We investigated the activity within the upstream regulatory region (URR) of the HPV types 8, 38, 92, 93 and 96, as well as their responsiveness to UV-B irradiation and cellular differentiation. Promoter activities were higher in HaCaT than in SiHa cells, corresponding to the HPV tissue tropism. Transcriptional start sites were mapped at P₉₂ (HPV-38), P₄₅ (HPV-92), P₇₄₃₉ (HPV-93) and P₂₅₆ (HPV-96). Transcription from HPV-8, 93 and 96 URR was up-regulated by cellular differentiation, linking the activity of these HPVs to the cellular state. UV-B irradiation activated HPV-8 but inhibited HPV-38 and HPV-93 whereas HPV-92 and 96 were not affected. As there are variable UV-B responses among the HPV types, further studies of interactions between UV-B and HPV need to consider the HPV type.