An LC-MS/MS method for determination of forsythiaside in rat plasma and application to a pharmacokinetic study
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- 作者：Geng-Nan Wang ; Rui-Le Pan ; Yong-Hong Liao ; Ying Chen ; Jing-Tian Tang ; Qi Chang
- 关键词：Forsythiaside ; LC-MS/MS ; Pharmacokinetics ; Bioavailability
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2010
- 出版时间：1 January 2010
- 年：2010
- 卷：878
- 期：1
- 页码：102-106
- 全文大小：337 K
文摘
A highly sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of forsythiaside in rat plasma using epicatechin as internal standard. The analytes were extracted by solid-phase extraction and chromatographied on a C18 column eluted with a gradient mobile phase of acetonitrile and water both containing 0.2 % formic acid. The detection was performed by negative ion electrospray ionization in multiple reaction monitoring mode, monitoring the transitions m/z 623 → 161 and m/z 289 → 109 for forsythiaside and epicatechin, respectively. The assay was linear over the concentration ranges of 2.0–50.0 and 50.0–5000.0 ng/mL with limits of detection and quantification of 0.2 and 1.0 ng/mL, respectively. The precision was <10.8 % and the accuracy was >91.9 % , and extraction recovery ranged from 81.3 % to 85.0 % . This method was successfully applied to a pharmacokinetic study of forsythiaside in rats after intravenous (20 mg/kg) and oral (100 mg/kg) administration, and the result showed that the compound was poorly absorbed with an absolute bioavailability being approximately 0.5 % .