An LC–MS/MS method for the simultaneous determination of chlorogenic acid, forsythiaside A and baicalin in rat plasma and its application to pharmacokinetic study of Shuang-huang-lian in rats
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- 作者：Jun-Xiao Ye ; Wang Wei ; Li-Hui Quan ; Chun-Yu Liu ; Qi Chang ; Yong-Hong Liao
- 关键词：LC– ; MS/MS ; Shuang-huang-lian ; Pulmonary delivery ; Traditional Chinese medicine
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2010
- 出版时间：1 August 2010
- 年：2010
- 卷：52
- 期：4
- 页码：625-630
- 全文大小：274 K
文摘
An LC–MS/MS method was developed for the simultaneous determination of chlorogenic acid, forsythiaside A and baicalin, three major ingredients in Shuang-huang-lian preparations, in rat plasma. Following extraction by methanol–ethyl acetate–trifluoroacetic acid (49:49:2, v/v/v), the extracted analytes were separated on a reverse phase C12 column using a gradient mobile phase system of acetonitrile–water containing 0.1 % formic acid. The limits of quantification were between 1.0 and 2.1 ng/mL, the precision was <7 % and the accuracy was between 94 % and 107 % . The validated method was applied to a comparative pharmacokinetic study in rats after administration of Shuang-huang-lian solutions via intravenous, peroral or intratracheal routes. The results showed that the three chemical markers were more rapidly and thoroughly absorbed following pulmonary delivery as compared with peroral administration.