Identification of Pediococcus acidilactici and Pediococcus pentosaceus based on 16S rRNA and ldhD gene-targeted multiplex PCR analysis
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- 作者：Mora ; Diego ; Fortina ; Maria Grazia ; Parini ; Carlo ; Manachini ; Pier Luigi
- 关键词：Pediococcus acidilactici ; Pediococcus pentosaceus ; Bacterial identification ; Polymerase chain reaction ; DNA probe
- 刊名：FEMS Microbiology Letters
- 出版年：1997
- 出版时间：June 15, 1997
- 年：1997
- 卷：151
- 期：2
- 页码：231-236
- 全文大小：419 K
文摘
A multiplex PCR assay that can readily and unambiguously identify Pediococcus acidilactici and pediococcus pentosaceus strains was developed to give an easy-to-read profile based on the amplification of a 16S rRNA gene fragment, specific for each species, and a d-lactate dehydrogenase gene fragment specific for Pediococcus acidilactici strains.