PCR-mediated site-directed mutagenesis on PedB gene and HaeIII restriction as a rapid tool for discrimination among pediocin AcH/PA-1 producer strains

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• 作者：Mora ; Diego ; Fortina ; Maria Grazia ; Parini ; Carlo ; Manachini ; Pier Luigi
• 刊名：Food Microbiology
• 出版年：2000
• 出版时间：August, 2000
• 年：2000
• 卷：17
• 期：4
• 页码：415-420
• 全文大小：150 K

文摘

Nucleotide sequences of amplified pedB genes from Pediococcus parvulus andLactobacillus plantarum pediocin AcH/PA-1 producer strains were performed. The obtained data were aligned with the published Pediococcus acidilactici pedB sequence, showing a single base substitution in the third codon position of a putative serine. A PCR-mediated site directed mutagenesis on pedB gene, followed by Hae III restriction analysis was then carried out with the aim to rapidly discriminate among pediocin AcH/PA-1 producer strains of different species.