Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with lymphedema-distichiasis syndrome (LDS) carrying an insertion-deletion complex mutation in the FOXC2 gene

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• 作者：Munenari Itoh^a ; ^{seafowl@jikei.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Shiho Kawagoe^a ; Hirotaka James Okano^b ; Hidemi Nakagawa^a
• 刊名：Stem Cell Research
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：16
• 期：3
• 页码：611-613
• 全文大小：741 K

文摘

Expanded human T cells from a Japanese male with lymphedema-distichiasis syndrome (LDS) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of established iPSC line, LDS-iPSC8, was confirmed by the expression of stem cell markers and the differentiation capability into three germ layers. LDS-iPSC8 may be a useful cell resource for the establishment of in vitro LDS modeling and the study for vascular and lymph vessel development.