Here, we showed the modulation of Flvcr1 gene expression in different tissues and cell lines in response to hypoxia. Chromatin immunoprecipitation analysis demonstrated that HIF2伪 and HIF-dependent transcription factor ETS1 (v-ets avian erythroblastosis virus E26 oncogene homolog 1) bind at the region 鈭?#xA0;318/+ 39 of the Flvcr1 promoter. Analysis of Caco2 cells in which HIF2伪 or ETS1 were silenced or overexpressed demonstrated that, both HIF2伪 and ETS1 are involved in the transcriptional regulation of Flvcr1a and that HIF2伪 is absolutely required for Flvcr1a induction upon hypoxia.
The inclusion of the Flvcr1 gene in the group of HIF2伪-responsive genes strengthens its role in hypoxia-stimulated processes like erythropoiesis, vasculogenesis and heme absorption.