Impact of asymmetrical flow field-flow fractionation on protein aggregates stability
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- 作者：Carmen R.M. Bria ; S. Kim Ratanathanawongs Williams ; krwillia@mines.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Field-Flow fractionation ; Protein aggregates stability ; Light scattering ; Shear stress ; Focusing ; Sample dilution
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：23 September 2016
- 年：2016
- 卷：1465
- 期：Complete
- 页码：155-164
- 全文大小：1746 K
文摘
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Stability of aggregate species is observed to be molar mass and size dependent.
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Syringe shear stress is not detectable using AF4-MALS, but is observed by batch-mode DLS.
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The focusing step, unique to AF4, does not impact anti-SA IgG aggregates stability.
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Dilution in AF4 is shown to be significantly lower than widely perceived.
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AF4 theory may provide more accurate sizes for weakly bound aggregates than online DLS.