Studies on the catalytic behaviour of a cholinesterase-like abzyme in an AOT microemulsion system
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- 作者：Franqueville ; Eric ; Stamatis ; Haralambos ; Loutrari ; Heleni ; Friboulet ; Alain ; Kolisis ; Fragiskos
- 关键词：Catalytic antibody ; Reverse micelle ; AOT surfactant ; Isooctane
- 刊名：Journal of Biotechnology
- 出版年：2002
- 出版时间：August 7, 2002
- 年：2002
- 卷：97
- 期：2
- 页码：177-182
- 全文大小：112 K
文摘
The hydrolytic activity of a monoclonal catalytic antibody (9A8) (abzyme) with acetylcholinesterase-like activity was investigated in water-in-oil (w/o) microemulsions (reverse micelles) based on sodium bis-2-(ethylhexyl)sulfosuccinate (AOT) in isooctane, using p- and o-nitrophenylacetate (p-and o-NPA) as substrates. The dependence of the abzyme hydrolytic activity on the molar ratio of water to surfactant (wo) showed a bell-shaped curve, presenting a maximum at wo