Selective disulfide reduction for labeling and enhancement of Fab antibody fragments
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- 作者：Terence L. Kirleya ; terry.kirley@uc.edu" class="auth_mail" title="E-mail the corresponding author ; Kenneth D. Greisb ; Andrew B. Normana
- 关键词：Monoclonal antibody ; Selective disulfide reduction ; Fab fragments ; TCEP agarose ; Sulfhydryl modification ; Antibody conjugation
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：25 November 2016
- 年：2016
- 卷：480
- 期：4
- 页码：752-757
- 全文大小：751 K
文摘
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TCEP agarose is effective for selective reduction of a single Fab disulfide bond.
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This disulfide is solvent accessible and distant from the antigen binding site.
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A variety of buffers of varying pHs can be used, simplifying subsequent steps.
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The methods used are simple, easily verifiable, reproducible, and quantitative.
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The selectively reduced Fab has many experimental and clinical applications.