Strategies for enhancing fermentative production of acetoin: A review

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• 作者：Zijun Xiao^a ; ^{zjxiao@upc.edu.cn" class="auth_mail} ; Jian R. Lu^b ; ^{j.lu@manchester.ac.uk" class="auth_mail}
• 关键词：Acetoin ; 2 ; 3-Butanediol ; Molecular regulation ; Bacterial fermentation ; Production enhancement methods ; Strain development ; Process optimization
• 刊名：Biotechnology Advances
• 出版年：March-April, 2014
• 年：2014
• 卷：32
• 期：2
• 页码：492-503
• 全文大小：841 K

文摘

Acetoin is a volatile compound widely used in foods, cigarettes, cosmetics, detergents, chemical synthesis, plant growth promoters and biological pest controls. It works largely as flavour and fragrance. Since some bacteria were found to be capable of vigorous acetoin biosynthesis from versatile renewable biomass, acetoin, like its reduced form 2,3-butanediol, was also classified as a promising bio-based platform chemical. In spite of several reviews on the biological production of 2,3-butanediol, little has concentrated on acetoin. The two analogous compounds are present in the same acetoin (or 2,3-butanediol) pathway, but their production processes including optimal strains, substrates, derivatives, process controls and product recovery methods are quite different. In this review, the usages of acetoin are reviewed firstly to demonstrate its importance. The biosynthesis pathway and molecular regulation mechanisms are then outlined to depict the principal network of functioning in typical species. A phylogenetic tree is constructed and the relationship between taxonomy and acetoin producing ability is revealed for the first time, which will serve as a useful guide for the screening of competitive acetoin producers. Genetic engineering, medium optimization, and process control are effective strategies to improve productivity as well. Currently, downstream processing is one of the main barriers in efficient and economical industrial acetoin fermentation. The future prospects of microbial acetoin production are discussed in light of the current progress, challenges, and trends in this field.