Vulnerability to DNA damage in the aging rat substantia nigra: a study with the comet assay

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• 作者：Giovannelli ; Lisa ; Decorosi ; Francesca ; Dolara ; Piero ; Pulvirenti ; Luigi
• 关键词：Disorders of the nervous system ; Basal ganglia ; Substantia nigra ; Parkinson’ ; s disease ; Aging ; Oxidative stress ; DNA
• 刊名：Brain Research
• 出版年：2003
• 出版时间：April 18, 2003
• 年：2003
• 卷：969
• 期：1-2
• 页码：244-247
• 全文大小：142 K

文摘

Oxidative DNA damage was measured in the substantia nigra (SN), cortex, hippocampus, striatum and hypothalamus of 3- and 24-month-old rats, using single-cell gel electrophoresis (SCGE, ‘comet’ assay) which allows the detection of DNA breaks and oxidized bases. A significant increase in basal DNA damage was selectively found in the SN of aged rats. FPG-sensitive oxidative DNA damage was also significantly increased in the SN of aged rats and, to a lesser extent, in the cortex and hypothalamus. These data show a higher vulnerability of SN to oxidative damage with aging and indicate that the detection of DNA damage within discrete brain nuclei can provide a reliable tool for investigating oxidative damage in neurodegenerative processes.