Highly electroconductive multiwalled carbon nanotubes as potentially useful tools for modulating calcium balancing in biological environments

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• 作者：Annalucia Serafino ; PhD^a ; Anna Rita Togna ; PhD^b ; Giuseppina I. Togna ; PhD^b ; Antonella Lisi ; PhD^a ; Mario Ledda ; PhD^a ; Settimio Grimaldi ; PhD^a ; Julie Russier ; PhD^b ; Federica Andreola ; BS^a ; Marc Monthioux ; PhD^c ; Francois Bé ; guin ; PhD^d ; Massimo Marcaccio ; PhD^e ; Stefania Rapino ; PhD^e ; Francesco Paolucci ; PhD^e ; Silvana Fiorito ; MD^a ; ^{silvana.fiorito@ift.cnr.it}
• 关键词：Carbon nanotubes ; MWCNTs ; Calcium balancing ; Intracellular Ca2+ mobilization
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：8
• 期：3
• 页码：299-307
• 全文大小：1666 K

文摘

Aiming to explore the mechanisms modulating cell-carbon nanotube interactions, we investigated whether Ca²⁺ ion balancing between intra- and extracellular environments could be affected by multiwalled carbon nanotubes (MWCNTs). We analyzed the effects induced by two different kinds of MWCNTs (as prepared and annealed at 2400¡ãC) on the intracellular Ca²⁺ ion levels in rat electrically sensitive cells and on the intercellular junction integrity of rat adenocarcinoma colon cells and platelet aggregation ability, which depend on the Ca²⁺ concentration in the medium. MWCNTs, purified by annealing and more electroconductive as compared to nonannealed MWCNTs, affected Ca²⁺ ion balancing between extra- and intracellular environments and induced changes on Ca²⁺ ion-dependent cellular junctions and platelet aggregation, behaving as the calcium chelator ethylene glycol tetraacetic acid. This could be due to the sorption of cationic Ca²⁺ ions on CNTs surface because of the excess of negatively charged electrons on the aromatic units formed on MWCNTs after annealing.

From the Clinical Editor

The authors investigated whether Ca²⁺ ion balance between intra- and extracellular space can be modulated by multiwalled carbon nanotubes (MWCNTs). Annealed nanotubes induced changes on Ca²⁺ dependent cellular junctions and platelet aggregation, behaving similary to ethylene glycol tetraacetic acid, an established calcium chelator.