- 作者：Luca Arcaini ; Silvia Zibellini ; Francesco Passamonti ; Sara Rattotti ; Marco Lucioni ; Rosangela Invernizzi ; Michele Merli ; Silvia Rizzi ; Emanuela Boveri ; Elisa Rumi ; Cesare Astori ; Cristina Picone ; Marz
- 关键词：Immunoglobulin heavy chain ; Mutational status ; Splenic marginal zone lymphoma ; Hepatitis C virus
- 刊名：Blood Cells, Molecules, and Diseases
- 出版年：2009
- 出版时间：May-June 2009
- 年：2009
- 卷：42
- 期：3
- 页码：286-291
- 全文大小：189 K
Abstract
Immunoglobulin gene usage and somatic mutation patterns were studied in 59 patients with splenic marginal zone lymphoma and were correlated with clinical characteristics.Fifty-nine IGHV rearrangements were amplified. IGHV1, IGHV3, and IGHV4 subgroups accounted for 30 % , 56 % , and 14 % of sequences, respectively. IGHV genes most frequently used were IGHV1-2 (n = 12), IGHV3-23 (n = 15), IGHV3-30 (n = 7) and IGHV4-34 (n = 5). IGHV was unmutated in 25 % .
Villous lymphocytes > 10 % were detected in 50 % of patients belonging to the IGHV1-2 group, in 21 % of the IGHV3-23 group, and in no patient of the IGHV3-30 group (p = 0.05). Liver involvement was present in 50 % of the IGHV3-30 group, in 9 % of the IGHV3-23 group, and in no patient of the IGHV1-2 group (p = 0.04). HCV-serology was positive in 50 % of the IGHV3-30 group, in 7 % of the IGHV3-23 group, and in 17 % of the IGHV1-2 group (p = 0.04). The proportion of intermediate and high risk patients according to the SMZL score was higher in the unmutated respect to the mutated group (69 % vs 32 % , p = 0.05).
In conclusion, IGHV rearrangement analysis in splenic marginal zone B-cell lymphoma reveals a non-random preference for use of IGHV1-2, IGHV3-23 and IGHV3-30 genes, whose presence differs according to clinical features and prognostic category.