Clast4, the murine homologue of human eIF4E-Transporter, is highly expressed in developing oocytes and post-translationally modified at meiotic maturation

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• 作者：J. Carlos Villaescusa ; Patrick Allard ; Emanuela Carminati ; Maria Kontogiannea ; Daniela Talarico ; Francesco Blasi ; Riaz Farookhi and Arturo C. Verrotti
• 关键词：Ovary ; Follicular development ; Cup ; eIF4E ; Translation initiation
• 刊名：Gene
• 出版年：2006
• 出版时间：15 February 2006
• 年：2006
• 卷：367
• 期：Complete
• 页码：101-109
• 全文大小：615 K

文摘

In metazoans, translational regulation of a set of maternal mRNAs directs oocyte maturation and early embryogenesis. These transcripts are often kept dormant until their products are spatially and temporally required in development. The interaction between general translation factors (i.e. eIF4E) and their specific interactors influences translation initiation. A search of the protein database for a mouse homologue of the Drosophila Cup protein, a translational repressor during female germ-line development, identified the product of the Clast4 gene. In this report, we show that Clast4 mRNA and protein are highly expressed within the cytoplasm of growing oocytes. The Clast4 protein is stable during this developmental window and post-translationally modified by phosphorylation upon oocyte meiotic maturation. Additionally, we show that Clast4 and eIF4E directly interact by means of a canonical and functional eIF4E-binding motif. Our results suggest that Clast4, similar to Drosophila Cup, may act at the translational level during murine female germ-line development.