Casein modified gold nanoparticles for future theranostic applications

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• 作者：Marisol Espinoza-Casta&#241 ; eda^a ; Alfredo de la Escosura-Mu&#241 ; iz^a ; Gemma Gonzá ; lez-Ortiz^b ; Susana M. Martí ; n-Orú ; e^b ; José ; Francisco Pé ; rez^b ; Arben Merkoç ; i^a ; ^c ; ^{arben.merkoci@icn.cat}
• 关键词：Gold nanoparticle ; Biosensor ; Bacteria ; Caseinglycopeptide ; Caseinglycomacropeptide ; Hydrogen evolution electrocatalysis ; Theranostic
• 刊名：Biosensors and Bioelectronics
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：40
• 期：1
• 页码：271-276
• 全文大小：468 K

文摘

The synthesis and characterization of gold nanoparticles (AuNPs 20 nm sized) modified with k-casein derived peptides in order to monitor the peptide effect as bacterial adhesion inhibitor, thanks to the carrier/concentrator effect of the AuNPs is here presented. Some aspects related to the stability of AuNP/peptide conjugates for a potential application in the design of an electrochemical biosensor for pathogen bacteria detection are also discussed. This peptide based nanoparticle assay takes advantage of the dual character of the AuNPs: as carrier of the biorecognition molecule and also as electrocatalytic label, allowing the evaluation of the pathogen bacteria-peptide interaction in a simple and rapid way through the chronoamperometric monitoring of the hydrogen evolution reaction on screen-printed carbon electrodes. The developed proof of concept theranostic system may open the way to therapeutic and biosensing applications with interest for various fields.