EcPV-2 is transcriptionally active in equine SCC but only rarely detectable in swabs and semen from healthy horses

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• 作者：Sabine Sykora^a ; Lisa Samek^b ; ¹ ; Katharina Sch&ouml ; nthaler^b ; ¹ ; Franziska Palm^c ; Giuseppe Borzacchiello^d ; Christine Aurich^e ; Sabine Brandt^b ; ^{sabine.brandt@vetmeduni.ac.at}
• 关键词：Horse ; SCC ; Semen ; Swabs ; Milk ; Papillomavirus ; EcPV-2 ; mRNA ; DNA
• 刊名：Veterinary Microbiology
• 出版年：2012
• 出版时间：6 July, 2012
• 年：2012
• 卷：158
• 期：1-2
• 页码：194-198
• 全文大小：368 K

文摘

Squamous cell carcinomas (SCC) are malignant tumours arising from keratinocytes. In horses, there is increasing evidence for m>Equus caballusm> papillomavirus type 2 (EcPV-2) being causally involved in SCC development. However, only little is known regarding intralesional transcription of the virus, and sparse information on the incidence of EcPV-2 infection in healthy equids is available so far.

Using RT-PCR, total mRNA from 8 EcPV-2 DNA-positive and 1 EcPV-2 negative SCC/SCC precursor lesions was screened for the presence of EcPV-2 E6 and E1 transcripts. Using PCR, we tested 193 sample specimens (30 ocular swabs, 94 genital swabs, 54 semen and 15 milk samples) from a total of 161 apparently healthy horses for the presence of EcPV-2 genes E7 and E6 or E2. Positive results were confirmed by repeating the PCR reactions, and by amplicon sequencing.

E6 mRNA was detectable in 8/8 EcPV-2 DNA-positive lesions, whereas only 3/8 scored positive for E1 mRNA. EcPV-2 PCR scored positive for DNA from 1/30 ocular swabs, 4/94 genital swabs, 0/54 semen and 0/15 milk samples, thus resulting in an overall detection rate of 5/193, m>i.e.m> 2.6 % . The demonstrated presence of viral mRNA in all EcPV-2 DNA-positive lesions is suggestive for an active pathogenic role of the virus in SCC development. This finding and the low incidence of EcPV-2 DNA in healthy equids further strengthen the concept of an aetiologic association of EcPV-2 with equine SCC disease.