Ipomoea asarifolia neutralizes inflammation induced by Tityus serrulatus scorpion venom

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• 作者：Maira Conceiç ; ã ; o Jerô ; nimo de Souza Lima^a ; Mariana Angé ; lica Oliveira Bitencourt^a ; Allanny Alves Furtado^a ; Hugo Alexandre Oliveira Rocha^b ; Ruth Medeiros Oliveira^b ; Arnó ; bio Antô ; nio da Silva-Jú ; nior^a ; Eryvaldo Só ; crates Tabosa do Egito^c ; Denise Vilarinho Tambourgi^d ; Silvana Maria Zucolotto^e ; Matheus de Freitas Fernandes-Pedrosa^a ; ^{mpedrosa@ufrnet.br" class="auth_mail}
• 关键词：AE ; aqueous extract ; BF ; butanol fraction ; CH2Cl2 ; dichloromethane ; CH2O2 ; formic acid ; CH3OH ; methanol ; COBEA ; Brazilian Committee for Animal Experimentation ; DF ; dichloromethane fraction ; DMEM ; Dulbecco壮s modified Eagle壮s medium ; EAF ; ethyl acetate fraction ; EtOAc ; ethyl acetate ; H2O ; Water ; i.p. ; intraperitoneal ; i.v. ; intravenous ; IFN-纬 ; Interferon 纬 ; IL-12 ; interleukin 12 ; IL-1伪 ; interleukin 1伪 ; IL-1尾 ; interleukin 1尾 ; IL-6 ; interleukin 6 ; MTT ; 3-(4 ; 5-dimethythiazol-2-yl)-2 ; 5-diphenyl-2H-tet
• 刊名：Journal of Ethnopharmacology
• 出版年：14 May 2014
• 年：2014
• 卷：153
• 期：3
• 页码：890-895
• 全文大小：1023 K

文摘

Envenoming caused by scorpion sting is a serious public health problem. In Brazil, 13,038 accidents caused by venomous animals have been reported. Of this total, 53% of the cases and 14 deaths were caused by scorpions. Furthermore, Tityus serrulatus (Buthidae) is the most dangerous scorpion due to the high toxicity of its venom. The treatment is the common supportive therapy and the serum therapy, but some people do not have access to both therapies and seek healing through the use of medical plants.

Aim of the study

This study evaluated the ability of the crude extract and fractions from the leaves of Ipomoea asarifolia in neutralizing the main biological effects caused by Tityus serrulatus envenoming in mice.

Materials and methods

BALB/c mice were pretreated (i.v.) with 100 渭位 of aqueous extracts and fractions dichloromethane, ethyl acetate, and n-butanol (CH₂Cl₂, EtOAc, and n-BuOH, respectively) of Ipomoea asarifolia, rutin or saline. Then, the animals received 100 渭位 (i.p.) of venom of Tityus serrulatus (0.8 mg/kg). After six hours, the peritoneal lavage was performed with PBS and the number cells were determined using a Neubauer chamber. The supernatants were collected for determination of cytokines, such as IL-6, IL-12, and IL-1尾.

Results

The aqueous extract, fractions and rutin, at all doses, significantly reduced cell migration, which was endorsed by the reduction of the levels of certain cytokines.

Conclusion

This is the first study that demonstrated the potential effect of Ipomoea asarifolia against inflammation caused by Tityus serrulatus venom, suggesting that these extracts and/or their bioactive molecules, especially the flavonoid rutin, have potential use in the therapy of this envenomation.