Functional expression of the fractalkine (CX₃C) receptor and its regulation by lipopolysaccharide in rat microglia

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• 作者：Boddeke ; Erik W.G.M. ; Meigel ; Inge ; Frentzel ; Stefan ; Biber ; Knut ; Renn ; Li Q. ; Gebicke-Hä ; rter ; Peter
• 关键词：Fractalkine ; CX₃C₁ receptor ; Lipopolysaccharide
• 刊名：European Journal of Pharmacology
• 出版年：1999
• 出版时间：June 18, 1999
• 年：1999
• 卷：374
• 期：2
• 页码：309-313
• 全文大小：176 K

文摘

Functional expression of CX₃CR1, a recently discovered receptor for the chemokine fractalkine, was investigated in cultured rat microglia. Reverse transcriptase polymerase chain reaction (PCR) experiments show abundant expression of fractalkine receptor mRNA in microglia. mRNA expression of fractalkine was undetectable in astrocytes and microglia but was very strong in cortical neurons. Incubation of microglia with lipopolysaccharide (100 ng/ml) transiently suppressed expression of fractalkine receptor mRNA. Fractalkine induced a concentration-dependent (10⁻¹⁰–10⁻⁸ M) and, at high concentrations, oscillatory mobilization of intracellular Ca²⁺ in microglia The concentration–response curve of fractalkine was shifted to the right after 12 h incubation with lipopolysaccharide. It is concluded that treatment with endotoxin downregulates expression of fractalkine receptor mRNA in rat microglia and suppresses the functional response to fractalkine.