Generation and characterization of quinolone-specific DNA aptamers suitable for water monitoring
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- 作者：C. Reinemann ; christine.reinemann@ufz.de" class="auth_mail" title="E-mail the corresponding author ; U. Freiin von Fritsch1 ; S. Rudolph2 ; B. Strehlitz
- 关键词：Aptamer ; SELEX ; Antibiotic ; Quinolone ; Water monitoring
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 March 2016
- 年：2016
- 卷：77
- 期：Complete
- 页码：1039-1047
- 全文大小：1318 K
文摘
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Aptamer selection by Capture-SELEX procedure.
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Structure switching aptamers with high specificity for several quinolones.
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KD values determined to be in the low nanomolar range (KD=0.1–56.9 nM).
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Aptamer functionality in real water samples was demonstrated.
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Selected aptamers suitable for biosensor applications in environmental analytics.