Cloning and characterization of metallothionein gene (HcMT) from Halostachys caspica and its expression in E. coli
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- 作者：Zhongyuan Liu1 ; Hongen Meng1 ; Hasiyatihan Abdulla ; Fuchun Zhang ; Xinfang Mao ; lilyxjmm@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：qRT-PCR ; quantitative real time PCR ; HcMT ; Halostachys caspica metallothionein ; IPTG ; isopropyl β-d-thiogalactoside ; OD ; optical density ; Trx ; thioredoxin protein
- 刊名：Gene
- 出版年：2016
- 出版时间：10 July 2016
- 年：2016
- 卷：585
- 期：2
- 页码：221-227
- 全文大小：1251 K
文摘
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A Type 2 metallothionein (HcMT) gene was cloned from H. caspica.
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HcMT gene was up-regulated under Cu2 +, Zn2 + and Cd2 + stress in H. caspica.
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TrxA-HcMT expressed in E. coli could enhance the tolerance of bacteria to Cu2 +, Zn2 + and Cd2 +.
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Purified TrxA-HcMT fusion protein expressed in E. coli has the ability to bind Cu2 +, Zn2 +, and Cd2 +.