Interaction of stem cells with nano hydroxyapatite-fucoidan bionanocomposites for bone tissue regeneration

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• 作者：Ahn Tae Young^a ; Jeong Han Kang^a ; Dong Jun Kang^a ; Jayachandran Venkatesan^b ; ^c ; Hee Kyung Chang^d ; Ira Bhatnagar^e ; Kwan-Young Chang^f ; Jae-Ho Hwang^g ; Ziad Salameh^h ; Se-Kwon Kim^b ; ^{sknkim@pknu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Hui Taek Kim^a ; ; Dong Gyu Kimⁱ ;
• 关键词：Hydroxyapatite ; Fucoidan ; Biomaterials ; Bone tissue engineering
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：93
• 期：part_PB
• 页码：1488-1491
• 全文大小：1423 K

文摘

The combination of bioceramics with biopolymers are playing major role in the construction of artificial bone. Hydroxyapatite (HA) has been extensively studied as a material in bone repair and replacement in last two decades. In the present study, we have prepared the hydroxyapatite-fucoidan (HA-Fucoidan) nanocomposites by in situ chemical method and biologically characterized them for bone graft substitute. Biological results inferred that mineralization effect of HA-F nanocomposites shows significant enhancement compared to HA in adipose derived stem cell (ADSC). It may be due to the addition of fucoidan in the nanocomposites. The important gene expression such as osteocalcin, osteopontin, collagen and runx-2 were checked using ADSC with HA and HA-fucoidan nanocomposites and the results show that the enhancements were found at 7th day. Furthermore, we have performed in vivo study of HA-fucoidan nanocomposites with rabbit model and a slight amount of bone formation was observed in HA-fucoidan nanocomposites. Herewith, we suggest that HA-fucoidan nanocomposites will be good biomaterials for bone repair/replacement in future.