Purification, expression and characterization of a novel α-l-fucosidase from a marine bacteria Wenyingzhuangia fucanilytica
详细信息 查看全文
- 作者：Shujun Dong ; Yaoguang Chang ; changyg@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jingjing Shen ; Changhu Xue ; Feng Chen
- 关键词：Fucosidase ; Marine bacterium ; Alf1_Wf ; GH29 ; Fucoidan
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：9-17
- 全文大小：1141 K
文摘
- •
A new α-l-fucosidase (Alf1_Wf) was purified from marine bacterium W. fucanilytica.
- •
Alf1_Wf was successfully expressed in E. coli and annotated as a GH29-A fucosidase.
- •
It was the first reported GH29-A bacterial fucosidase containing CBM domain.
- •
Alf1_Wf can act on pNP-fucose and slightly on α1,4-fucosidic linkage.
- •
It fails to degrade the fucoidan polysaccharide but can hydrolyze fucoidan fragment.