A Markov chain model for N-linked protein glycosylation - towards a low-parameter tool for model-driven glycoengineering
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- 作者：Philipp N. Spahna ; c ; Anders H. Hansend ; Henning G. Hansend ; Johnny Arnsdorfd ; Helene F. Kildegaardd ; Nathan E. Lewisb ; c ; nlewisres@ucsd.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：GlcNAc ; N-acetylglucosamine ; GalT ; β-1-4-galactosyltransferase ; Fut8 ; α-1-6-fucosyltransferase 8 ; GnTIV ; N-acetylglucosamine-transferase IV ; CHO ; Chinese hamster ovary ; EPO ; erythropoietin ; mAb ; monoclonal antibody ; IgG ; immunoglobulin
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：33
- 期：Complete
- 页码：52-66
- 全文大小：2555 K
文摘
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A non-kinetic low-parameter Markov model for N-glycosylation is presented.
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10">The model is fit to experimental glycoprofiles.
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15">The model can be used to predict the effect of glycosyltransferase knock-downs.
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We show validation data on IgG, EPO and the secretome.