Human milk oligosaccharides are important ingredients for health-related nutrition.
10">Their production via multistep enzymatic cascades and using whole cells is reviewed.
15">Recombinant production and properties of prokaryotic fucosyltransferases are summarized.
Enzymatic fucosylation integrated with metabolism for GDP-l-fucose supply is described.
Current bottlenecks of fucosylated HMO production are discussed.