Drugs interacting with organic anion transporter-1 affect uptake of Tc-99m-mercaptoacetyl-triglycine (MAG3) in the human kidney: Therapeutic drug interaction in Tc-99m-MAG3 diagnosis of renal function and possible application of Tc-99m-MAG3 for drug devel

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• 作者：Noriko Takahara^a ; Tsuneo Saga^b ; Masayuki Inubushi^b ; Hiroyuki Kusuhara^c ; Chie Seki^b ; Sumito Ito^c ; Nobuyuki Oyama^a ; Osamu Yokoyama^a ; Yuichi Sugiyama^d ; Yasuhisa Fujibayashi^b ; ^{yfuji@nirs.go.jp}
• 关键词：MAG3 ; Organic anion transporter ; OAT1 ; Drug interaction ; Kidney
• 刊名：Nuclear Medicine and Biology
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：40
• 期：5
• 页码：643-650
• 全文大小：1740 K

文摘

Introduction

Renal uptake of Tc-99m-MG3 involves organic anion transporter (OAT). Treatment with drugs showing OAT affinity might interfere with renal uptake of Tc-99m-MAG3, leading to misinterpretation in Tc-99m-MAG3. This study was conducted to discuss a possible drug interference with Tc-99m-MAG3 diagnosis on OAT sites.

Methods

Renal uptake and plasma clearance of Tc-99m-MAG3 were analyzed in healthy volunteers under control and OAT1 and OAT3 related drug treatment conditions. An in vitro uptake study using OAT1 or OAT3 expressing cells was also conducted.

Results

Both PAH and probenecid treatment induced delays in Tc-99m-MAG3 clearance from blood, and reductions in the renal uptake clearance. As a result, the normalized effective renal plasma flow estimated from Tc-99m-MAG3 clearance was significantly underestimated, whereas the glomerular filtration rate estimated from plasma creatinine levels was unchanged. The transport activity of Tc-99m-MAG3 was higher in OAT1-expressing cells than in OAT3-expressing cells.

Conclusion

Drugs with OAT1 affinity affect the renal uptake of Tc-99m-MAG3 and blood clearance. This might cause misinterpretation of functional diagnosis of the kidney using Tc-99m-MAG3.