Targeted mutagenesis of an odorant receptor co-receptor using TALEN in Ostrinia furnacalis
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- 作者：Bin Yang ; Takeshi Fujii ; Yukio Ishikawa ; Takashi Matsuo ; matsuo@utlae.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：Genome editing ; Knock out ; Lepidoptera ; Mutant ; Non-model insect
- 刊名：Insect Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：70
- 期：Complete
- 页码：53-59
- 全文大小：1361 K
文摘
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TALEN-mediated mutagenesis was performed for the first time in a non-model pest insect, Asian corn borer Ostrinia furnacalis.
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Mutants of the gene encoding odorant receptor co-receptor (Orco) were obtained successfully.
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In the mutant moths, antennal response to pheromones was decreased to a trace level, and behavioral responses were abolished.