Influence of the culture medium on the production of nitric oxide and expression of inducible nitric oxide synthase by activated macrophages in vitro
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- 作者：Tomoya Kawakami1 ; Kana Kawamura1 ; Ko Fujimori ; Atsushi Koike ; Fumio Amano ; amano@gly.oups.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Macrophage activation ; F-12 ; DMEM ; Nitric oxide ; Tumor necrosis factor-α ; Interleukin-1β
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：5
- 期：Complete
- 页码：328-334
- 全文大小：1059 K
文摘
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Activation macrophages phenotypes were different in DMEM and F-12 containing FBS.
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NO production and iNOS expression by LPS and IFN-γ were remarkably different.
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-generating activity, and production TNF-α and IL-1β were also different.- •
The choice of culture medium is important for estimation of macrophage activation.