Monitoring of the multiple bacteriocin production by Enterococcus faecium NKR-5-3 through a developed liquid chromatography and mass spectrometry-based quantification system

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• 作者：Rodney H. Perez ; Kohei Himeno ; Naoki Ishibashi ; Yoshimitsu Masuda ; Takeshi Zendo ; Koji Fujita ; Pongtep Wilaipun ; Vichien Leelawatcharamas ; Jiro Nakayama ; Kenji Sonomoto
• 关键词：Lactic acid bacteria ; Multiple bacteriocin ; Bacteriocin quantification ; Electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS) ; Induction
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：114
• 期：5
• 页码：490-496
• 全文大小：404 K

文摘

Enterococcus faecium NKR-5-3 produces four antimicrobial peptides referred here as enterocins NKR-5-3A, B, C and D. A two-step electrospray ionization-liquid chromatography and mass spectrometry (ESI-LC/MS)-based quantification system was developed to monitor its multiple bacteriocin production profiles, which is essential in understanding the complex production regulation mechanism of strain NKR-5-3. The developed ESI-LC/MS-based quantification system can easily monitor the multiple bacteriocin production of this strain. Using the developed system, the production of enterocin NKR-5-3B was found to be not as variable as those of the other enterocins in different cultivation media. Production of enterocin NKR-5-3B was also found to have a wider optimum incubation temperature (20-30¡ãC) than enterocins NKR-5-3A, C and D (25¡ãC). Furthermore, at least 2?nM of the bacteriocin-like inducing peptide, enterocin NKR-5-3D, regulated the production of NKR-5-3 enterocins except enterocin NKR-5-3B. These findings taken together suggest that enterocin NKR-5-3B has an independent production regulation mechanism from the other NKR-5-3 enterocins. The developed system could effectively pin-point the production profiles of the multiple bacteriocins of E.?faecium NKR-5-3 under different fermentation conditions.