The influence of restraint stress on the expression of mRNAs for IL-6 and the IL-6 receptor in the hypothalamus and midbrain of the rat
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- 作者：Shizuya ; Koji ; Komori ; Teruhisa ; Fujiwara ; Ryoichi ; Miyahara ; Satoru ; Ohmori ; Masaki ; Nomura ; Junichi
- 关键词：interleukin-6 ; interleukin-6 receptor ; stress ; mRNA ; hypothalamus ; midbrain ; reverse transcriptase-polymerase chain reaction
- 刊名：Life Sciences
- 出版年：1997
- 出版时间：August 1, 1997
- 年：1997
- 卷：61
- 期：10
- 页码：PL135-PL140
- 全文大小：326 K
文摘
Using the reverse transcriptase-polymerase chain reaction (RT-PCR), we investigated the influence of restraint stress on the expression of the mRNA for interleukin-6 (IL-6) and the mRNA for the IL-6 receptor (IL-6R) in the rat brain. After rats had been restrained for 4 hours, the hypothalamus and midbrain were removed at fixed intervals up to 24 hours, and levels of IL-6 mRNA and of IL-6R mRNA in these regions were determined by RT-PCR. Restraint stress significantly enhanced the expression of IL-6 mRNA and reduced that of IL-6R mRNA in the midbrain, whereas the stress caused the reduced expression of IL-6R mRNA without any change in the level of IL-6 mRNA in the hypothalamus. After the stress, the expression of mRNAs for IL-6 and IL-6R continued to diminish in both regions. These findings indicate that the levels of mRNAs for both of IL-6 and IL-6R in the rat brain can be influenced by restraint stress.