Effects of restraint stress on α₁ adrenoceptor mRNA expression in the hypothalamus and midbrain of the rat

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• 作者：Miyahara ; Satoru ; Komori ; Teruhisa ; Fujiwara ; Ryoichi ; Shizuya ; Koji ; Yamamoto ; Masato ; Ohmori ; Masaki ; et. al.
• 关键词：α ; ₁ Adrenoceptor ; Stress ; mRNA ; Hypothalamus ; Midbrain ; Reverse transcriptase&ndash ; polymerase chain reaction ; Hypothalamic&ndash ; pituitary&ndash ; adrenal axis
• 刊名：Brain Research
• 出版年：1999
• 出版时间：October 2, 1999
• 年：1999
• 卷：843
• 期：1-2
• 页码：130-135
• 全文大小：391 K

文摘

We examined the effects of restraint stress on α₁ adrenoceptor mRNA expression in the rat brain using reverse transcriptase–polymerase chain reaction (RT-PCR). After rats had been restrained for 10, 30, 60, 120 or 240 min, the hypothalamus and midbrain were removed immediately and α₁ adrenoceptor mRNA levels in these regions were determined by RT-PCR. Blood samples were also collected for simultaneous measurement of serum adrenocorticotropic hormone (ACTH) and corticosterone. Restraint stress resulted in a variety of changes in the hypothalamus and midbrain. In the hypothalamus, 30 and 60 min of stress resulted in a significant fall in the level of α₁ adrenoceptor mRNA relative to the control. This was associated with a rise in serum ACTH and corticosterone. In the midbrain, significant elevation of α₁ adrenoceptor mRNA was noted after 60, 120 and 240 min of restraint stress. Our findings indicated that the influence of restraint stress on α₁ adrenoceptor mRNA level in the hypothalamus is different to that of the midbrain region in rats.