A Saccharomyces cerevisiae gene essential for viability has been conserved in evolution

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• 作者：Rinaldi ; Teresa ; Bolotin-Fukuhara ; Monique ; Frontali ; Laura
• 关键词：Yeast mitochondrion ; tRNA processing ; mutant ; revertant ; nuclear-mitochondrial interaction ; endonuclease ; respiration
• 刊名：Gene
• 出版年：1995
• 出版时间：July 4, 1995
• 年：1995
• 卷：160
• 期：1
• 页码：135-136
• 全文大小：158 K

文摘

To identify the gene coding for the endonuclease which processes the 3′ end of mitochondrial (mt) tRNA transcripts in Saccharomyces cerevisiae, nuclear mutations able to complement a mt mutant (Ts932) defective for this process were isolated and analyzed. One of these mutants exhibited a growth defect both on respiratory and fermentable media. Complementation of this phenotype with a S. cerevisiae centromeric wild-type genomic library has allowed us to identify a new essential S. cerevisiae gene strongly conserved in various eukaryotic organisms.