Elevated expression of mature miR-21 and miR-155 in cancerous gastric tissues from Chinese patients with gastric cancer

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• 作者：Li Liu^a ; ^b ; Qi Chen^a ; ^{qichen@njmu.edu.cn} ; Rensheng Lai^b ; Xiaobin Wu^b ; Xiaoyu Wu^c ; Fukun Liu^c ; Guohua Xu^d ; Yong Ji^a
• 关键词：MicroRNA ; expression ; gastric cancer
• 刊名：Journal of Biomedical Research
• 出版年：2010
• 出版时间：May 2010
• 年：2010
• 卷：24
• 期：3
• 页码：187-197
• 全文大小：881 K

文摘

Objective

MicroRNA (miRNA) expression is deregulated in many types of human cancers. We sought to investigate the expression patterns of the miRNAs, miR-21, miR-145 and miR-155 in sporadic gastric cancer in a Chinese population.

Methods

Total RNA was extracted from archived gastric cancer tissues and adjacent non-cancerous tissues from 20 pairs of paraffin-embedded specimens. Expression levels of miR-21, miR-145 and miR-155 were detected by quantitative reverse transcriptase PCR using a specific stem-loop primer, with U6 as the internal reference gene.

Results

The expression of miR-21 and miR-155 in gastric cancer samples was significantly higher than in paired non-cancerous samples (P < 0.05). There was no statistically significant difference in expression levels of miR-145 between cancerous and non-cancerous tissues (P > 0.05).

Conclusion

In Chinese sporadic gastric cancer tissues, the expressions of the oncogenic miR-21 and miR-155 were significantly up-regulated, while the expression of the tumor suppressor miR-145 was decreased, although this decrease was not statistically significant. Thus there is specificity in the miRNA expression pattern in gastric cancers in the Chinese population.