Myclobutanil worsens nonalcoholic fatty liver disease: An in vitro study of toxicity and apoptosis on HepG2 cells
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- 作者：Antonietta Stellavatoa ; 1 ; Monica Lambertib ; 1 ; monica.lamberti@unina2.it" class="auth_mail" title="E-mail the corresponding author ; Anna Virginia Adriana Pirozzia ; Francesca de Novellisa ; Chiara Schiraldia
- 关键词：HepG2 ; hepatocarcinoma cell line ; LDH ; intracellular lactate dehydrogenase ; MTT ; tetrazolium dye 3-(4 ; 5-dimethylthiazol-2-yl)-2 ; 5-diphenyltetrazolium bromide ; FAs ; fatty acids (oleic 18 ; 1 and linoleic acid 18 ; 2 ratio 1 ; 1 ; v/v) ; ARfD ; acute reference dose ; NOEL ; no-observed-effect level ; MFO ; mixed function oxidase ; MOE ; margins of exposure
- 刊名：Toxicology Letters
- 出版年：2016
- 出版时间：16 November 2016
- 年：2016
- 卷：262
- 期：Complete
- 页码：100-104
- 全文大小：668 K
文摘
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Hepatic toxicity of myclobutanil exacerbates fatty acid-induced steatosis.
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Myclobutanil reduces viability of HepG2 cells to <50% at 100 ppm and to <10% at 500 ppm.
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Myclobutanil modifies the function of enzymes such LDH and cytochrome c.
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Analysis of biomarkers such as Bcl-xL/Bak and Mcl-1/Bak confirmed activation of cell death pathways at low doses of myclobutanil.