Epithelioid and sarcomatoid mesothelioma cells were inoculated subcutaneously and intrapleurally into nude mice. Biodistribution and PET imaging studies were conducted by injecting [18F]fluoro-2-deoxy- In epithelioid mesothelioma models, biodistribution experiments showed that tumor uptake of [11C]S-dThd was significantly higher than that of [18F]FDG. On the other hand, in sarcomatoid models, [18F]FDG showed significantly higher accumulation than the other two tracers. These differential uptakes of the three tracers were confirmed by PET imaging. The cellular uptake of [14C]FDG and [3H]FLT and TK1 activity in sarcomatoid cells were higher than those of epithelioid cells. GLUT-1 protein was strongly expressed in sarcomatoid but not in epithelioid tumor. We observed a high percentage of Ki-67-positive cells in both epithelioid and sarcomatoid tumors. We established nude mouse models of epithelioid and sarcomatoid subtypes of mesothelioma. PET tracers applicable for the evaluation of epithelioid and sarcomatoid mesothelioma would vary: [18F]FLT and [11C]S-dThd seemed suitable for the epithelioid subtype and [18F]FDG seemed suitable for the sarcomatoid subtype in our mouse models. Our results indicated that cellular uptake and TK1 activity in vitro are not always consistent with tracer uptake of [18F]FLT and [11C]S-dThd in vivo. These mouse models and PET imaging might be useful tools for evaluating new and effective treatments in mesothelioma.Results
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