Licochalcone A significantly suppresses LPS signaling pathway through the inhibition of NF-κB p65 phosphorylation at serine 276
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- 作者：Jun-ichi Furusawa ; Megumi Funakoshi-Tago ; Kenji Tago ; Tadahiko Mashino ; Hideo Inoue ; Yoshiko Sonoda ; Tadashi Kasahara
- 关键词：Licochalcone A ; LPS ; NO ; NF-κ ; B ; Phosphorylation
- 刊名：Cellular Signalling
- 出版年：2009
- 出版时间：May 2009
- 年：2009
- 卷：21
- 期：5
- 页码：778-785
- 全文大小：1213 K
文摘
Licorice root, Glycyrrhiza inflata, has been used as a traditional medicine for the treatment of bronchial asthma and inflammation; however, the mechanism of its anti-inflammatory activity has not been clarified. Here, we investigated the effect of Licochalcone A, a major component of G. inflata, on the LPS signaling pathway. We found that Licochalcone A remarkably inhibited LPS-induced NO production, and TNFα expression and MCP-1 expression in both RAW264.7 cells and primary macrophages. Furthermore, when injected with Licochalcone A prior to injection of LPS, the serum level of TNFα and MCP-1 in C57BL/6 mice was clearly decreased, indicating that Licochalcone A has a potent anti-inflammatory effect both in vitro and in vivo. Strikingly, Licochalcone A significantly inhibited LPS-induced NF-κB transcriptional activation; however, it had no effect on not only the phosphorylation and degradation of IκBα but also nuclear translocation and DNA binding activity of NF-κB p65. Interestingly, Licochalcone A markedly inhibited the phosphorylation of p65 at serine 276. As a result, it reduced NF-κB transactivation by preventing the interaction of p65 with p300. Taken together, Licochalcone A might contribute to the potent anti-inflammatory effect of G. inflata through the unique mechanism of NF-κB inhibition.