Identification of candidate genes involved in endogenous protection mechanisms against acute pancreatitis in mice

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• 作者：Shinji Nakada ; Koichi Tsuneyama ; Ichiro Kato ; Yoshiaki Tabuchi ; Ichiro Takasaki ; Yukihiro Furusawa ; Hiroshi Kawaguchi ; Makoto Fujimoto ; Hirozo Goto ; Hiroaki Hikiami ; Takashi Kondo ; Yasuo Takano ; Yutak
• 关键词：Pancreatitis ; Caerulein ; GeneChip ; Claudin ; Heat shock protein ; Protease inhibitor
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：15 January 2010
• 年：2010
• 卷：391
• 期：3
• 页码：1342-1347
• 全文大小：472 K

文摘

We surveyed changes of the gene expression profile in caerulein-exposed pancreas using Affymetrix GeneChip system (39,000 genes). Up-regulation of genes coding for claudin 4, claudin 7, F11 receptor, cadherin 1, integrin beta 4, syndecan 1, heat shock proteins b1/90aa1, Serpinb6a, Serpinb6b, Serpinb9, Bax, Bak1, calpain 2, calpain 5, microtubule-associated protein 1 light chain 3 alpha, S100 calcium-binding proteins A4/A10 were found in mouse pancreas exposed to caerulein for 12 h. In contrast, the anti-apoptotic gene Bcl2 was down-regulated. The functions of these genes concern tight junction formation, cell–cell/cell–matrix adhesions, stress response, protease inhibition, apoptosis, autophagy, and regulation of cytoskeletal dynamics. Caerulein-exposed pancreatic acinar cells were immunohistochemically stained for claudin 4, cadherin 1, integrin beta 4, heat shock protein b1, and Serpinb6a. In conclusion, we have newly identified a set of genes that are likely to be involved in endogenous self-protection mechanisms against acute pancreatitis.