Spontaneous mutation 7B-1 in tomato impairs blue light-induced stomatal opening

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• 作者：Jan Hlavinka^a ; ^{hlavinka.jan@gmail.com} ; Jan Nau&scaron ; ^a ; ^{naus@prfnw.upol.cz} ; Martin Fellner^b ; ^{martin.fellner@upol.cz}
• 关键词：9-AC ; anthracene-9-carboxylic acid ; ABA ; abscisic acid ; BL ; blue light ; FC ; fusicoccin ; NIF ; niflumic acid ; PAR ; photosynthetically active radiation ; RL ; red light ; SE ; standard error ; WL ; white light ; WT ; wild type
• 刊名：Plant Science
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：209
• 期：Complete
• 页码：75-80
• 全文大小：1214 K

文摘

It was reported earlier that 7B-1 mutant in tomato (Solanum lycopersicum L.), an ABA overproducer, is defective in blue light (BL) signaling leading to BL-specific resistance to abiotic and biotic stresses. In this work, we examine responses of stomata to blue, red and white lights, fusicoccin, anion channel blockers (anthracene-9-carboxylic acid; 9-AC and niflumic acid; NIF) and ABA. Our results showed that the aperture of 7B-1 stomata does not increase in BL, suggesting that 7B-1 mutation impairs an element of BL signaling pathway involved in stomatal opening. Similar stomatal responses of 7B-1 and wild type (WT) to fusicoccin or 9-AC points out that activity of H⁺-ATPase and 9-AC-sensitive anion channels per se is not likely affected by the mutation. Since 9-AC restored stomatal opening of 7B-1 in BL, it seems that 9-AC and BL could block similar type of anion channels. The stomata of both genotypes did not respond to NIF neither in darkness nor in any light conditions tested. In light, 9-AC but not NIF restored stomatal opening inhibited by ABA in WT and 7B-1. We suggest that in comparison to WT, the activity of S-type anion channels in 7B-1 is more promoted by increased ABA content, and less reduced by BL, because of the mutant resistance to BL.