Regulation of differentiation by a PHD domain in the NUP98-PHF23 fusion protein

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• 作者：Jocelyn C. Reader ; Qixin Leng ; Feyruz V. Rassool ; Yi Ning
• 关键词：Differentiation ; NUP98-PHF23 fusion ; PHD domain
• 刊名：Leukemia Research
• 出版年：2010
• 出版时间：August 2010
• 年：2010
• 卷：34
• 期：8
• 页码：1094-1097
• 全文大小：1981 K

文摘

Acute myeloid leukemia (AML) is frequently associated with chromosomal translocations. These translocations produce specific fusion genes that play crucial roles in leukemogenesis. We recently identified a novel NUP98-PHF23 fusion in AML. In this study, we attempt to determine the role of NUP98-PHF23 protein and its plant homeodomain (PHD) and coiled-coil domain in regulation of cellular differentiation and protein distribution. We provide evidence that NUP98-PHF23, through its PHD domain, impairs TPA-induced differentiation of K562 cells. While the fusion protein localizes to the nucleus, its deletion mutant without the PHD domain resides exclusively in the nucleolus, suggesting a potential link between chromatin-binding PHD domain and nuclear architecture.