Metabolic flux analyses of Pseudomonas aeruginosa cystic fibrosis isolates
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- 作者：Michael J. Oppermana ; b ; Yair Shachar-Hilla ; yairhill@msu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：CCE ; Carbon conversion efficiency ; CF ; Cystic Fibrosis ; EDP ; Entner-Doudoroff Pathway ; EMPP ; Embden Meyerhof Parnas Pathway ; FBA ; Flux balance analysis ; FVA ; Flux Variability Analysis ; HCA ; Hierarchical Clustering Analysis ; MFA ; Metabolic flux analysis ; OPPP ; Oxidative Pentose Phosphate Pathway ; PCA ; Principal Component Analysis ; SSres ; sum of squared residuals ; TCA ; Tricarboxylic acid cycle
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：38
- 期：Complete
- 页码：251-263
- 全文大小：1599 K
文摘
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MFA of five P. aeruginosa CF isolates demonstrate two metabolic phenotypes.
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One metabolic phenotype is a high TCA and EDP flux with low OPPP flux.
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One metabolic phenotype is a high OPPP flux with low TCA flux.
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FBA demonstrates suboptimal growth of P. aeruginosa CF isolates.
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13C fingerprinting has lower resolution then MFA in determining differences.