Advance chromatin extraction improves capture performance of protein A affinity chromatography
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- 作者：Rui Nian ; Wei Zhang ; Lihan Tan ; Jeremy Lee ; Xeuzhi Bi ; Yuansheng Yang ; Hui Theng Gan ; Pete Gagnon ; pete_gagnon@bti.a-star.edu.sg" class="auth_mail" title="E-mail the corresponding author ; pete@validated.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Protein A ; IgG purification ; Chromatin extraction ; Loading residence time ; Ligand leakage ; Cleaning efficiency
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：29 January 2016
- 年：2016
- 卷：1431
- 期：Complete
- 页码：1-7
- 全文大小：981 K
文摘
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Extraction of chromatin from cell harvest improved protein A performance.
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Chromatin extraction increased capacity 10–15% across all tested media.
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Chromatin extraction reduced ligand leakage by half.
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HCP contamination was reduced below 1 ppm, DNA below 1 ppb.
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Chromatin extraction permitted effective cleaning with 50 mM NaOH.