Advance chromatin extraction enhances performance and productivity of cation exchange chromatography-based capture of Immunoglobulin G monoclonal antibodies
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- 作者：Rui Nian ; Pete Gagnon ; pete_gagnon@mac.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Chromatin ; IgG ; Cation exchange capture ; Contaminant reduction ; Capacity ; Productivity
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：1453
- 期：Complete
- 页码：54-61
- 全文大小：1021 K
文摘
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Chromatin caused precipitation and loss of IgG during sample equilibration.
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It also depressed cation exchange capacity and IgG recovery.
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Advance chromatin extraction suspended these limitations.
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HCP was reduced to <1000 ppm, DNA to <1 ppb, and aggregates to <2.5%.
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A single polishing step reduced HCP to 15 ppm and aggregates to <0.1%.