Biological in situ characterization of polymeric microbubble contrast agents

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• 作者：Sha Wan^a ; Gabriella Egri^b ; Letizia Oddo^c ; Barbara Cerroni^c ; Lars Dä ; hne^b ; Gaio Paradossi^c ; Anna Salvati^a ; ^d ; Iseult Lynch^e ; Kenneth A. Dawson^a ; ^{kenneth.a.dawson@cbni.ucd.ie" class="auth_mail" title="E-mail the corresponding author} ; Marco P. Monopoli^a ; ^f ; ^{marcomonopoli@rcsi.ie" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Microbubbles ; Protein corona ; Flow cytometry ; Opsonin proteins ; Dysopsonin proteins ; Bionano interactions
• 刊名：International Journal of Biochemistry and Cell Biology
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：75
• 期：Complete
• 页码：232-243
• 全文大小：4028 K

文摘

Polymeric microbubbles (MBs) are gas filled particles composed of a thin stabilized polymer shell that have been recently developed as valid contrast agents for the combined use of ultrasonography (US), magnetic resonance imaging (MRI) and single photon emission computer tomography (SPECT) imaging. Due to their buoyancy, the commonly available approaches to study their behaviour in complex media are not easily applicable and their use in modern medicine requires such behaviour to be fully elucidated. Here we have used for the first time flow cytometry as a new high throughput approach that allows characterisation of the MB dispersion, prior to and after exposure in different biological media and we have additionally developed a method that allows characterisation of the strongly bound proteins adsorbed on the MBs, to fully predict their biological behaviour in biological milieu.