Enzyme, 尾-galactosidase immobilized on membrane surface for galacto-oligosaccharides formation from lactose: Kinetic study with feed flow under recirculation loop
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- 作者：Tapas Palai ; Avaneesh K. Singh ; Prashant K. Bhattacharya ; pkbhatta@iitk.ac.in" class="auth_mail
- 关键词：BCA ; bicinchoninic acid ; FESEM ; field-emission scanning electron microscopy ; GOS ; galacto-oligosaccharides ; HFMC ; hollow fiber membrane contactor ; IEMR ; immobilized enzymatic membrane reactor ; ILC ; initial lactose concentration ; ONP ; o-nitro phenol ; ONPG ; o-nitrophenyl-尾-d-galactopyranoside ; PVDF ; polyvinylidene fluoride
- 刊名：Biochemical Engineering Journal
- 出版年：15 July 2014
- 年：2014
- 卷：88
- 期：Complete
- 页码：68-76
- 全文大小：1506 K
文摘
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Enzymatic conversion of lactose into galacto-oligosaccharides with 尾-galactosidase.
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尾-Galactosidase immobilized on PVDF membrane with feed under recirculation mode.
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A maximum GOS yield of 30% was observed with 50 g/L initial lactose concentration.
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Prediction out of 5-step, 9-parameter developed model showed excellent matching.
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Immobilized state enzyme on membrane showed better stability than its native state.