Multiple Conformations of Gal3 Protein Drive the Galactose-Induced Allosteric Activation of the GAL Genetic Switch of Saccharomyces cerevisiae

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• 作者：Rajesh Kumar Kar¹ ; Hungyo Kharerin ; Ranjith Padinhateeri ; Paike Jayadeva Bhat ; ^{jayadeva@iitb.ac.in}
• 关键词：KNF ; Koshland&ndash ; Nemethy&ndash ; Filmer ; MWC ; Monod&ndash ; Wyman&ndash ; Changeux ; Gal3p ; Gal3 protein ; MD ; molecular dynamics ; d.o. ; drop out ; gly/lac ; glycerol plus lactate ; 2-DG ; 2-deoxy-galactose ; Gal1p ; galactokinase ; ORF ; open reading frame ; epPCR ; error-prone PCR ; EtBr ; ethidium bromide ; SDM ; site-directed mutagenesis ; FACS ; fluorescence-activated cell sorting ; CMD ; conventional MD ; TMD ; targeted MD ; SASA ; solvent-accessible surface area ; PCA ; principal component analysis ; PC1 ; first princi
• 刊名：Journal of Molecular Biology
• 出版年：2017
• 出版时间：6 January 2017
• 年：2017
• 卷：429
• 期：1
• 页码：158-176
• 全文大小：2541 K
• 卷排序：429

文摘

Gal3p activates GAL genetic switch of Saccharomyces cerevisiae. Mechanism of allosteric transition of Gal3p is proposed. A rare super-open conformation of Gal3p is observed. Residues that stabilize super-open conformation are identified. Conformational selection in Gal3p drives the activation of GAL genetic switch.